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Detection of equine arteritis virus by real-time reverse transcription-PCR assay



Detection of equine arteritis virus by real-time reverse transcription-PCR assay



Chinese Journal of Veterinary Science 25(2): 141-144



A one-step fluorogenic RT-PCR (5'-nuclease probe-based) assay using a primer/TaqMan probe set designed against a highly conserved region of open reading frame 7 (ORF7) of EAV genome was developed for the specific detection of EAV. The assay had an analytical sensitivity of 5 molecules of EAV RNA allowing the detection of EAV in clinical samples or tissue culture fluid (TCF) containing about 5 PFU/ micro litre.

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