EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Detection of nucleolar organizer regions on chromosomes by fluorescence in situ hybridization with human 28S rRNA gene and cloning of 28S rRNA gene in Sika deer


Animal Science Journal 75(2): 111-116
Detection of nucleolar organizer regions on chromosomes by fluorescence in situ hybridization with human 28S rRNA gene and cloning of 28S rRNA gene in Sika deer
The number and loci of nucleolar organizer regions (NOR) on chromosomes in Sika deer (Cervus nippon centralis) were determined by fluorescence in situ hybridization with a human 28S ribosomal RNA (rRNA) gene as a probe. Sika deer that live in Nikko National Park and its neighboring areas (Asio and Seta) in Japan were used. All of the analyzed metaphases had three or four NOR at the end of the first and second longest telocentric autosomes. Nucleolar organizer region association, which is associated specifically on parts of NOR between chromosomes, was also observed clearly. A Sika deer 28S rRNA gene was produced by a polymerase chain reaction method. The nucleotide sequence of a Sika deer 28S rRNA gene determined by an automatic sequencer was 97 bp, and showed homogeneity of 88% for the human sequence.


Accession: 004099321

DOI: 10.1111/j.1740-0929.2004.00164.x



Related references

Detection of nucleolar organizer regions on chromosomes by flourescence in situ hybridization with human 28S rRNA gene and cloning of 28S rRNA gene in Sika deer. Animal Science Journal il; 75(2): 111-116, 2004

Combined light and fluorescent microscopical imaging of nucleolar organizer regions and cellular rRNA as detected by fluorescent in situ hybridization. Cytometry 19(2): 171-176, 1995

Relation between the intensity of transcription and the rRNA gene content of individual nucleolar-forming regions of human chromosomes. Biulleten' Eksperimental'noi Biologii i Meditsiny 105(1): 63-65, 1988

Simultaneous fluorescence in situ hybridization of mRNA and rRNA for the detection of gene expression in environmental microbes. Methods in Enzymology 397: 352-371, 2005

Detection of WWE2-related Lentisphaerae by 16S rRNA gene sequencing and fluorescence in situ hybridization in landfill leachate. Canadian Journal of Microbiology 56(10): 846-852, 2010

Nitrifying bacterial communities in an aquaculture wastewater treatment system using fluorescence in situ hybridization (FISH), 16S rRNA gene cloning, and phylogenetic analysis. Biotechnology and Bioengineering 97(4): 985-990, 2006

Observations on rRNA gene replication in Chara vulgaris evaluated by in situ rRNA-DNA hybridization. Journal of cell science 88(3): 407-414, 1987

Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment. Genome 37(6): 1018-1021, 1994

Identification of pathogenic Nocardia species by reverse line blot hybridization targeting the 16S rRNA and 16S-23S rRNA gene spacer regions. Journal of Clinical Microbiology 48(2): 503-511, 2010

Physical localization of rRNA genes by two-colour fluorescent in-situ hybridization and sequence analysis of the 5S rRNA gene in Phalaris coerulescens. Hereditas 126(3): 289-294, 1997