+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Effect of different forms of alkali treatment on specific fermentation inhibitors and on the fermentability of lignocellulose hydrolysates for production of fuel ethanol

Effect of different forms of alkali treatment on specific fermentation inhibitors and on the fermentability of lignocellulose hydrolysates for production of fuel ethanol

Journal of Agricultural and Food Chemistry 50(19): 5318-5325

ISSN/ISBN: 0021-8561

PMID: 12207468

DOI: 10.1021/jf025565o

Treatment with alkali, particularly overliming, has been widely used as a method for the detoxification of lignocellulose hydrolysates prior to ethanolic fermentation. However, the mechanisms behind the detoxification effect and the influence of the choice of cation have not been well understood. In this study, a dilute acid hydrolysate of spruce and an inhibitor cocktail consisting of six known inhibitors were used to investigate different alkali detoxification methods. The various treatments included the addition of calcium hydroxide, sodium hydroxide, potassium hydroxide, and ammonia to pH 10.0 and subsequent adjustment of the pH to 5.5 with either sulfuric or hydrochloric acid as well as treatment with the corresponding amounts of calcium, sodium, and potassium as sulfate or chloride salts at pH 5.5. An RP-HPLC method was developed for the separation of 18 different inhibitors in the hydrolysate, including furaldehydes and phenolics. Detection and quantification were carried out by means of UV, DAD, and ESI-MS in negative mode. Treatment of the spruce hydrolysate with alkali resulted in up to approximately 40% decrease in the concentration of furaldehydes. The effects on the aromatic compounds were complex. Furthermore, SFE was performed on the precipitate formed during alkali treatment to evaluate the inhibitor content of the precipitate, and the following RP-HPLC analysis implied that potential inhibitors were removed mainly through conversion rather than through filtration of precipitate. Parallel experiments in which sulfuric acid or hydrochloric acid was used for acidification to pH 5.5 after alkali treatment indicated that the choice of anion did not affect the removal of inhibitors. Detoxification with calcium hydroxide and ammonia resulted in better fermentability using Saccharomyces cerevisiae than detoxification with sodium hydroxide. The results from the experiments with the inhibitor cocktail indicated that the positive effects of alkali treatment are difficult to explain by removal of the inhibitors only and that possible stimulatory effects on the fermenting organism warrant further attention.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 004120408

Download citation: RISBibTeXText

Related references

A study of three strategies for improving the fermentability of sugarcane bagasse hydrolysates for fuel ethanol production. International Sugar Journal 109(1297): 33-35, 37-39, 2007

Biotechnological strategies to overcome inhibitors in lignocellulose hydrolysates for ethanol production: review. Critical Reviews in Biotechnology 31(1): 20-31, 2011

Production of ethanol of other fuel molecules by anaerobic fermentation in fixed cell reactors for lignocellulose gasification. Energy from Biomass. Contractors' Meeting 1: 318-322, 1987

Effect of byproducts in lignocellulose hydrolysates on ethanol fermentation by Issatchenkia orientalis. Sheng Wu Gong Cheng Xue Bao 30(5): 753-764, 2014

Identification of the major fermentation inhibitors of recombinant 2G yeasts in diverse lignocellulose hydrolysates. Biotechnology for Biofuels 14(1): 92, 2021

Co-production of 1,2,4-butantriol and ethanol from lignocellulose hydrolysates. Bioresource Technology 282: 433-438, 2019

Improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents. Bioresource Technology 102(2): 1254-1263, 2011

Development of a Saccharomyces cerevisiae strain with enhanced resistance to phenolic fermentation inhibitors in lignocellulose hydrolysates by heterologous expression of laccase. Applied and Environmental Microbiology 67(3): 1163-1170, 2001

Ethanol addition enhances acid treatment to eliminate Lactobacillus fermentum from the fermentation process for fuel ethanol production. Letters in Applied Microbiology 66(1): 77-85, 2018

Enhanced ethanol production from industrial lignocellulose hydrolysates by a hydrolysate-cofermenting Saccharomyces cerevisiae strain. Bioprocess and Biosystems Engineering 42(5): 883-896, 2019

Overexpressing CCW12 in Saccharomyces cerevisiae enables highly efficient ethanol production from lignocellulose hydrolysates. Bioresource Technology 337: 125487, 2021

Overexpression of SFA1 in engineered Saccharomyces cerevisiae to increase xylose utilization and ethanol production from different lignocellulose hydrolysates. Bioresource Technology 313: 123724, 2020

Simultaneous hydrolysis and fermentation of lignocellulose versus separated hydrolysis and fermentation for ethanol production. Romanian Biotechnological Letters 16(1): 106-112, 2011

Fermentation of lignocellulosic hydrolysates for ethanol production. Enzyme and Microbial Technology 18(5): 312-331, 1996

Scale-up of abatement of fermentation inhibitors from acid hydrolysates for efficient conversion to ethanol as biofuel. Journal of Chemical Technology and Biotechnology 91(6): 1826-1834, 2016