Effect of magnesium supplementation on oxidative modification of human low density lipoprotein

L.X.aoHua; Wang RuiShu

Acta Nutrimenta Sinica 25(4): 366-369


ISSN/ISBN: 0512-7955
Accession: 004123788

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The effects of magnesium on the oxidation of low density lipoprotein (LDL) were systematically investigated using two different oxidation systems. LDLs were isolated from pooled fresh sera obtained from healthy humans by ultracentrifugation. Oxidation of LDL was induced by adding Cu2+ or co-culturing with human umbilical vein endothelial cells. Conjugate diene was measured to assess the susceptibility of LDL to oxidation. The extent of LDL modification was determined by measuring the formation of thiobarbituric acid reaction substances (TBARS). The presence of magnesium resulted in a protracted lag phase at doses of 0.3, 0.6, 1.2 and 2.4 mmol/litre, as well as a decreased production of TBARS when LDL was oxidized by the addition of Cu2+, at doses of 0.3 and 0.6 mmol/litre. Treatment of LDL with magnesium (0.3, 0.6, 1.2 and 2.4 mmol/litre) reduced the production of TBARS during endothelial cell-mediated LDL oxidation (P<0.05). The results indicated that magnesium protects low density lipoproteins from oxidation.