EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Effect of water stress on lupin stem protein analysed by two-dimensional gel electrophoresis



Effect of water stress on lupin stem protein analysed by two-dimensional gel electrophoresis



Planta 221(5): 716-728



Lupinus albus plants can withstand severe drought stress and show signs of recovery 24 h after rewatering (RW). Two-dimensional gel electrophoresis was used to evaluate the effect of water deficit (WD) on the protein composition of the two components of the lupin stem (stele and cortex). This was performed at three distinct stress levels: an early stage, a severe WD, and early recovery. Protein characterisation was performed through mass spectrometric partial sequencing. Modifications in the protein expression were first noticed at 3 days of withholding water, when the plant water status was still unaffected but some decrease in the relative soil water content had already occurred. An increase in serine proteases, possibly associated with WD sensing, was an early alteration induced by WD. When the stress severity increased, a larger number of stem proteins were affected. Immunophilin, serine protease and cysteine protease (well-known components of animal sensing pathways) were some of these proteins. The simultaneous expression of proteases and protease inhibitors that reacted differently to the stress level and to RW was found. Although the level of protease inhibitors was significantly raised, RW did not cause de novo expression of proteins. Many amino acid sequences did not match known sequences of either protein or expressed sequence tag databases. This emphasises the largely unknown nature of stem proteins. Nevertheless, some important clues regarding the way the lupin plant copes with WD were revealed.

(PDF emailed within 0-6 h: $19.90)

Accession: 004129509

Download citation: RISBibTeXText

PMID: 15668768

DOI: 10.1007/s00425-004-1478-0



Related references

Effect of androgens on protein synthesis and secretion in various regions of the rat epididymis, as analysed by two-dimensional gel electrophoresis. Molecular and Cellular Endocrinology 29(3): 255-270, 1983

Serum protein polymorphism of Swiss pig breeds analysed by two-dimensional electrophoresis. Dissertation Abstracts International C, Worldwide 52(3): 370, 1991

Phosphorylated and other modified forms of eukaryotic ribosomal protein S3 analysed by two-dimensional gel electrophoresis. Biochemical Journal 189(2): 241-245, 1980

Effects of testosterone on protein synthesis in rat seminal vesicles analysed by two-dimensional gel electrophoresis. Molecular and Cellular Endocrinology 24(1): 85-101, 1981

Two-dimensional electrophoresis of thylakoid proteins from yellow lupin subjected to thermal and light stress. Biological Bulletin of Poznan 34(0): 5-15, 1997

Differential recovery of lupin proteins from the gluten matrix in lupin-wheat bread as revealed by mass spectrometry and two-dimensional electrophoresis. Journal of Agricultural and Food Chemistry 59(12): 6696-6704, 2011

Effect of bile salts stress on protein synthesis of Lactobacillus casei Zhang revealed by 2-dimensional gel electrophoresis. Journal of Dairy Science 93(8): 3858-3868, 2010

Computer-analysed two-dimensional gel electrophoresis of proteins. Trends in biochemical sciences 5(11): 281-283, 1980

Polypeptide changes induced by salt stress, water deficit, and osmotic stress in barley roots: a comparison using two-dimensional gel electrophoresis. Electrophoresis 9(11): 781-787, 1988

Dog pancreatic microsomal-membrane polypeptides analysed by two-dimensional gel electrophoresis. Biochemical Journal 217(1): 145-157, 1984

Amylase isoforms in the bile of patients with pancreaticobiliary malunion analysed by two-dimensional electrophoresis. Fudan Xuebao (Yixuekexueban) 32(1): 79-82, 2005

The influence of water stress on plant development, productivity and seed wholesomeness of sweet and bitter cultivars of blue lupin and white lupin. Natural Sciences ( 4): 63-73, 2000

Glucose-induced changes of multiple mouse islet proteins analysed by two-dimensional gel electrophoresis and mass spectrometry. Diabetologia 48(3): 477-485, 2005

Folate stress protein characterization in Escherichia coli using two dimensional gel electrophoresis. FASEB Journal 9(6): A1276, 1995

The effect of water stress on the composition of carbohydrates in yellow lupin seeds and triticale kernels. Acta Physiologiae Plantarum 21(3 Suppl ): 81, 1999