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Efficient production of polyploid plants via protoplast culture of Iris fulva

Efficient production of polyploid plants via protoplast culture of Iris fulva

Cytologia 69(3): 327-333

In protoplast culture of the Louisiana iris species, Iris fulva (2n=42), N6 medium supplemented with 1 mg 2,4-D/litre, 1 mg kinetin/litre, 200 mg casein hydrolysate/litre, 250 mg proline/litre, 0.3 M glucose and 2.5 g gellan gum/litre was suitable for cell division and colony formation. When formed colonies were transferred to plant growth regulator-free Murashige and Skoog's medium, 61 plant lines were regenerated via somatic embryogenesis.

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Accession: 004140894

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DOI: 10.1508/cytologia.69.327

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