Evaluation of a diagnostic algorithm using immunoglobulin M enzyme-linked immunosorbent assay to differentiate human West Nile Virus and St. Louis Encephalitis virus infections during the 2002 West Nile Virus epidemic in the United States
Martin, D.A.; Noga, A.; Kosoy, O.; Johnson, A.J.; Petersen, L.R.; Lanciotti, R.S.
Clinical and Diagnostic Laboratory Immunology 11(6): 1130-1133
ISSN/ISBN: 1071-412X PMID: 15539517 DOI: 10.1128/cdli.11.6.1130-1133.2004
A diagnostic algorithm was developed to differentiate between human infections of West Nile virus (WNV) and St. Louis encephalitis virus (SLEV) using positive-to-negative (P/N) ratios derived from the immunoglobulin M capture enzyme-linked immunosorbent assay (MAC-ELISA). To validate this algorithm, we tested 1,418 serum and cerebrospinal fluid (CSF) samples from confirmed WNV and SLEV infections collected during the WNV epidemic of 2002 in the United States. WNV P/N-to-SLEV P/N ratios (W/S ratios) were calculated and used to identify the infecting virus.