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Experimental transmission of sheep scrapie by intracerebral and oral routes to genetically susceptible Suffolk sheep in the United States

Hamir, A.N.; Kunkle, R.A.; Richt, J.A.; Miller, J.M.; Cutlip, R.C.; Jenny, A.L.

Journal of Veterinary Diagnostic Investigation Official Publication of the American Association of Veterinary Laboratory Diagnosticians Inc 17(1): 3-9

2005

ISSN/ISBN: 1040-6387
PMID: 15690944
DOI: 10.1177/104063870501700103
Accession: 004156440
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Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. Susceptibility to the disease is partly dependent on the genetic makeup of the host. This study documents clinicopathological findings and the distribution of abnormal prion proteins (PrPres) by immunohistochemical and Western blot techniques, in tissues of genetically susceptible sheep inoculated with US sheep scrapie agents. Four-month-old Suffolk lambs (QQ or HQ at codon 171) were inoculated (5 intracerebrally and 19 orally) with an inoculum (#13-7) consisting of a pool of scrapie-affected sheep brains. Intracerebrally inoculated animals were euthanized when advanced clinical signs of scrapie were observed.

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Related References

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Pattison, I.; Millson, G. 1961: Experimental Transmission of Scrapie to Goats and Sheep by the Oral Route Journal of Comparative Pathology and Therapeutics 71: 171-176
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Mammadova, N.; Cassmann, E.D.; Moore, S.J.; Nicholson, E.M.; Greenlee, J.J. 2020: Experimental inoculation of CD11c+ B1 lymphocytes, CD68+ macrophages, or platelet-rich plasma from scrapie-infected sheep into susceptible sheep results in variable infectivity Access Microbiology 2(9): Acmi 000155
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Dickinson, A.G.; Stamp, J.T. 1969: Experimental scrapie in Cheviot and Suffolk sheep Journal of Comparative Pathology 79(1): 23-26
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Vilette, D.; Madelaine, M.F.; Laude, H. 2000: Establishment of astrocyte cell lines from sheep genetically susceptible to scrapie In Vitro Cellular and Developmental Biology. Animal 36(1): 45-49
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Garza, M.ía.C.; Fernández-Borges, N.; Bolea, R.; Badiola, J.J.é; Castilla, J.ín.; Monleón, E. 2011: Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie Plos one 6(12): E27525