Expression analysis of dihydroflavonol 4-reductase genes involved in anthocyanin biosynthesis in purple grains of wheat

Liu MaoSen; Wang Fang; Dong YuXiu; Zhang XianSheng

Journal of Integrative Plant Biology 47(9): 1107-1114

2005


ISSN/ISBN: 1672-9072
DOI: 10.1111/j.1744-7909.2005.00148.x
Accession: 004157011

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Abstract
The grain colour of wheat is an important characteristic in crop production. Dihydroflavonol 4-reductase genes (DFR) encode the key enzyme dihydroflavonol 4-reductase, which is involved in the pigmentation of plant tissues. To investigate the molecular mechanism of anthocyanin deposition in grains of wheat, we determined the expression of the wheat DFR gene in purple grains of cv. Heimai 76. The DFR transcripts were localized in the seed coat of purple grains rather than in the pericarp, whereas anthocyanins were accumulated in both tissues of purple grains, suggesting that anthocyanin deposition was mainly regulated at the transcriptional level. Overexpression of the TaDFR-A gene in Arabidopsis thaliana showed that TaDFR-A was responsible for the pigmentation of A. thaliana plant tissues, indicating TaDFR-A gene has the same role in A. thaliana.

Expression analysis of dihydroflavonol 4-reductase genes involved in anthocyanin biosynthesis in purple grains of wheat