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Expression in Escherichia coli of the capsid protein of Watermelon mosaic virus and production of specific antiserum

Expression in Escherichia coli of the capsid protein of Watermelon mosaic virus and production of specific antiserum

Fitopatologia Brasileira 29(2): 215-219

A Watermelon mosaic virus (WMV)-specific, polyclonal antiserum was raised in rabbits by immunization with purified capsid protein expressed and purified from Escherichia coli cells. The cp gene was RT-PCR-amplified using specific oligonucleotides, and viral RNA extracted from concentrated particles as a template. The amplified fragment was cloned in pBLUESCRIPT KS+ and fully sequenced in order to confirm its identity and integrity.

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Accession: 004157137

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DOI: 10.1590/S0100-41582004000200017

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