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Expression of chitinase-encoding genes in Bacillus thuringiensis and toxicity of engineered B. thuringiensis subsp. aizawai toward Lymantria dispar larvae



Expression of chitinase-encoding genes in Bacillus thuringiensis and toxicity of engineered B. thuringiensis subsp. aizawai toward Lymantria dispar larvae



Current Microbiology 48(3): 175-181



Chitinase genes from Aeromonas hydrophila and Bacillus circulans No.4.1 were cloned into the plasmid pHY300PLK and designated as pHYA2 and pHYB43, respectively. Both plasmids were introduced into various strains of B. thuringiensis by electroporation. Plasmid pHYB43 was generally structurally stable, but showed lower segregational stability than pHYA2 in B. thuringiensis subsp. aizawai when grown under nonselective conditions.

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Accession: 004157385

Download citation: RISBibTeXText

PMID: 15057461

DOI: 10.1007/s00284-003-4119-0


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