Gambogic acid inhibits proliferation of human lung carcinoma SPC-A1 cells in vivo and in vitro and represses telomerase activity and telomerase reverse transcriptase mRNA expression in the cells
Wu, Z-Qiu.; Guo, Q-Long.; You, Q-Dong.; Zhao, L.; Gu, H-Yan.
Biological and Pharmaceutical Bulletin 27(11): 1769-1774
ISSN/ISBN: 0918-6158 PMID: 15516720 DOI: 10.1248/bpb.27.1769
We determined the in vivo and in vitro antitumour activities of gambogic acid (GA; from the resin of Garcinia hanburyi) and one of the possible mechanisms for its inhibitory activities. The in vivo antitumour activity of GA was evaluated by the relative tumour growth ratio (T/C) in nude mice, and in vitro inhibition of SPC-A1 cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and trypan blue exclusion assay. Telomere repeats amplification protocol-polymerase chain reaction (TRAP-PCR), ELISA and RT-PCR were used to quantitatively detect telomerase activity and the expression of human telomerase reverse transcriptase (hTERT) mRNA, respectively.