EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Gene discovery and gene expression in the rice blast fungus, Magnaporthe grisea: analysis of expressed sequence tags


Gene discovery and gene expression in the rice blast fungus, Magnaporthe grisea: analysis of expressed sequence tags



Molecular Plant-Microbe Interactions: Mpmi 17(12): 1337-1347



ISSN/ISBN: 0894-0282

PMID: 15597739

DOI: 10.1094/mpmi.2004.17.12.1337

Over 28,000 expressed sequence tags (ESTs) were produced from cDNA libraries representing a variety of growth conditions and cell types. Several Magnaporthe grisea strains were used to produce the libraries, including a nonpathogenic strain bearing a mutation in the PMK1 mitogen-activated protein kinase. Approximately 23,000 of the ESTs could be clustered into 3,050 contigs, leaving 5,127 singleton sequences. The estimate of 8,177 unique sequences indicates that over half of the genes of the fungus are represented in the ESTs. Analysis of EST frequency reveals growth and cell type-specific patterns of gene expression. This analysis establishes criteria for identification of fungal genes involved in pathogenesis. A large fraction of the genes represented by ESTs have no known function or described homologs. Manual annotation of the most abundant cDNAs with no known homologs allowed us to identify a family of metallothionein proteins present in M. grisea, Neurospora crassa, and Fusarium graminearum. In addition, multiply represented ESTs permitted the identification of alternatively spliced mRNA species. Alternative splicing was rare, and in most cases, the alternate mRNA forms were unspliced, although alternative 5' splice sites were also observed.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004172082

Download citation: RISBibTeXText

Related references

Sequence analysis and expression pattern of MGTA1 gene in rice blast pathogen Magnaporthe grisea. Journal of Zhejiang University. Science. B 6(8): 817-824, 2005

Global gene expression during nitrogen starvation in the rice blast fungus, Magnaporthe grisea. Fungal Genetics and Biology: Fg and B 43(9): 605-617, 2006

Enhanced resistance to the rice blast fungus Magnaporthe grisea conferred by expression of a cecropin A gene in transgenic rice. Planta 223(3): 392-406, 2006

Constitutive expression of the defense-related Rir1b gene in transgenic rice plants confers enhanced resistance to the rice blast fungus Magnaporthe grisea. Plant Molecular Biology 43(1): 59-66, 2000

Mapping avirulence gene in the rice blast fungus Magnaporthe grisea. Scientia Agricultura Sinica 33(3): 49-53, 2000

Regulation of the MPG1 hydrophobin gene in the rice blast fungus Magnaporthe grisea. Molecular Plant-Microbe Interactions: Mpmi 15(12): 1253-1267, 2002

Transposon impala, a novel tool for gene tagging in the rice blast fungus Magnaporthe grisea. Molecular Plant-Microbe Interactions: Mpmi 14(3): 308-315, 2001

A large-insert (130 kbp) bacterial artificial chromosome library of the rice blast fungus Magnaporthe grisea: genome analysis, contig assembly, and gene cloning. Fungal Genetics and Biology: Fg and B 21(3): 337-347, 1997

CDNA microarray analysis to identify genes preferentially expressed during appressorium formation in the rice blast fungus, Magnaporthe grisea. Phytopathology 91(6 Suppl): S87, 2001

Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell 5(11): 1575-1590, 1993

Identification of a putative vacuolar serine protease gene in the rice blast fungus, Magnaporthe grisea. Bioscience Biotechnology and Biochemistry 66(3): 663-666, 2002

Identification and Characterization of Mpg1, a Gene Involved in Pathogenicity from the Rice Blast Fungus Magnaporthe grisea. The Plant Cell 5(11): 1575-1590, 1993

Genetic analysis and molecular mapping of the avirulence gene PRE1, a gene for host-species specificity in the blast fungus Magnaporthe grisea. Genome 49(8): 873-881, 2006

Targeted gene disruption of the neuronal calcium sensor 1 homologue in rice blast fungus, Magnaporthe grisea. Bioscience Biotechnology and Biochemistry 67(3): 651-653, 2003

A novel ABC transporter gene ABC2 involved in multidrug susceptibility but not pathogenicity in rice blast fungus, Magnaporthe grisea. Pesticide Biochemistry and Physiology 81(1): 13-23, 2005