+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Gibberellin is essentially required for carrot (Daucus carota L.) somatic embryogenesis: dynamic regulation of gibberellin 3-oxidase gene expressions



Gibberellin is essentially required for carrot (Daucus carota L.) somatic embryogenesis: dynamic regulation of gibberellin 3-oxidase gene expressions



Bioscience Biotechnology and Biochemistry 67(11): 2438-2447



A GA biosynthesis inhibitor, uniconazole, caused many shrunken embryos when it was supplied to cultured carrot (Daucus carota L.) cells at the induction of somatic embryos. The abnormality was prevented by exogenous GA(1) or GA(4). To analyze the status of GA biosynthesis during somatic embryogenesis, expression patterns of newly isolated genes encoding GA biosynthetic enzymes, two GA 20-oxidases, three GA 3-oxidases, and two GA 2-oxidases were observed by using a semi-quantitative reverse-transcription-polymerase chain reaction with gene-specific primers. Transcript levels of GA 20-oxidases and GA 2-oxidases did not change greatly during development of the somatic embryo. On the other hand, drastic changes were found in three GA 3-oxidase genes. Strikingly, expression of a GA 3-oxidase gene, DcGA3ox2, was elevated once in somatic embryogenesis, but not in the non-induced suspension cells. The enzymatic functions of these gene products were also confirmed using recombinant proteins expressed in Escherichia coli. Our results indicate that GA biosynthesis is required for carrot somatic embryogenesis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004177497

Download citation: RISBibTeXText

PMID: 14646205

DOI: 10.1271/bbb.67.2438


Related references

Occurrence of delta 1 10 gibberellin a 1 counterpart gibberellin a 1 gibberellin a 4 and gibberellin a 7 in somatic cell embryo cultures of carrot daucus carota and anise pimpinella anisum. Agricultural & Biological Chemistry 43(8): 1793-1794, 1979

Quantitation of gibberellins and the metabolism of 3H hydrogen ion gibberellin A1 during somatic embryogenesis in carrot and anise cell cultures Daucus carota, Pimpinella anisum. p. 1982

Quantitation of gibberellins and the metabolism of tritium labeled gibberellin a 1 during somatic embryogenesis in carrot daucus carota and anise pimpinella anisum cell cultures. Planta (Heidelberg) 155(5): 369-376, 1982

Metabolism of (3H) tritium gibberellin A4 in somatic suspension cell cultures of carrot Daucus carota. 1983

Metabolism of tritium labeled gibberellin a 4 in somatic suspension cell cultures of carrot daucus carota. Phytochemistry 22(7): 1585-1590, 1983

A chromo box gene from carrot (Daucus carota L.): its cDNA structure and expression during somatic and zygotic embryogenesis. Biochimica et Biophysica Acta, Gene Structure and Expression 1398(1): 42-46, 1998

Involvement of gibberellin and cytokinin in the formation of embryogenic cell clumps in carrot (Daucus carota). Journal of Plant Physiology 160(2): 133-141, 2003

Investigations on gibberellin and cytokinin activity in carrot roots at various developmental stages Daucus carota.1. Zeitschrift fur Pflanzenernahrung und Bodenkunde = Journal of plant nutrition and soil science 145(3): 268-277, 1982

Investigations on gibberellin and cyto kinin activity in carrot daucus carota roots at various developmental stages. Zeitschrift fuer Pflanzenernaehrung und Bodenkunde 145(3): 268-277, 1982

Somatic embryogenesis and artificial seed in carrot (Daucus carota L.). Somatic embryogenesis and synthetic seed 182, 1995

Localization of calcium during somatic embryogenesis of carrot (Daucus carota L.). Protoplasma 190(1/2): 107-118, 1996

Imaging of polarity during zygotic and somatic embryogenesis of carrot (Daucus carota L.). 1993

Studies on nitrogen metabolism during somatic embryogenesis in carrot Daucus carota. Plant tissue culture 1982: proceedings 5th International Congress of Plant Tissue and Cell Culture held at Tokyo and Lake Yamanake Japan July 11-16-1982 edited by Akio Fujiwara: 116, 1982

Nitrate reductase (E.C. 1.6.6.1) activity in somatic embryogenesis of carrot, Daucus carota L. Agro Ciencia 15(1): 27-32, 1999

Bioreactor studies of the effect of medium pH on carrot (Daucus carota L.) somatic embryogenesis. Plant Cell, Tissue and Organ Culture 36(2): 205-209, 1994