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Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze



Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze



Brazilian Archives of Biology and Technology 47(6): 873-879



A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were approximately 2.4 when respiring on succinate as substrate.

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Accession: 004196276

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