Section 5
Chapter 4,197

Improved technique for counting chromosomes in almond

Martinez Gomez, P.; Sanchez Perez, R.; Vaknin, Y.; Dicenta, F.; Gradziel, T.M.

Scientia Horticulturae 105(1): 139-143


ISSN/ISBN: 0304-4238
DOI: 10.1016/j.scienta.2005.01.019
Accession: 004196371

In almond (Prunus dulcis (Mill.) D.A. Webb), there are few chromosome studies. In this work, different methods described for slide preparation and staining of chromosomes in other Prunus species have been evaluated using almond root tips. By modifying and updating traditional methods, an improved technique for counting chromosomes has been developed. Root tips were first placed in cold water for 4 h at 0 degrees C and treated with 0.2% colchicine for 3 h at 5 degrees C.

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