+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vitro and in vivo inhibition of Helicobacter pylori by Lactobacillus casei strain Shirota



In vitro and in vivo inhibition of Helicobacter pylori by Lactobacillus casei strain Shirota



Applied and environmental microbiology 70(1): 518-526



We studied the potential inhibitory effect of Lactobacillus casei strain Shirota (from the fermented milk product Yakult [Yakult Ltd., Tokyo, Japan]) on Helicobacter pylori by using (i) in vitro inhibition assays with H. pylori SS1 (Sydney strain 1) and nine H. pylori clinical isolates and (ii) the in vivo H. pylori SS1 mouse model of infection over a period of 9 months. In vitro activity against H. pylori SS1 and all of the clinical isolates was observed in the presence of viable L. casei strain Shirota cells but not in the cell-free culture supernatant, although there was profound inhibition of urease activity. In vivo experiments were performed by oral administration of L. casei strain Shirota in the water supply over a period of 9 months to 6-week-old C57BL/6 mice previously infected with H. pylori SS1 (study group; n = 25). Appropriate control groups of H. pylori-infected but untreated animals (n = 25) and uninfected animals given L. casei strain Shirota (n = 25) also were included in the study. H. pylori colonization and development of gastritis were assessed at 1, 2, 3, 6, and 9 months postinfection. A significant reduction in the levels of H. pylori colonization was observed in the antrum and body mucosa in vivo in the lactobacillus-treated study group, as assessed by viable cultures, compared to the levels in the H. pylori-infected control group. This reduction was accompanied by a significant decline in the associated chronic and active gastric mucosal inflammation observed at each time point throughout the observation period. A trend toward a decrease in the anti-H. pylori immunoglobulin G response was measured in the serum of the animals treated with lactobacillus, although this decrease was not significant.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004197582

Download citation: RISBibTeXText

PMID: 14711683

DOI: 10.1128/aem.70.1.518-526.2004


Related references

In vitro inhibition of Helicobacter pylori by Lactobacillus casei strain shirota. Microbial Ecology in Health & Disease 12(2): 122, 2000

Anti-inflammatory activity of the probiotic Lactobacillus casei strain Shirota in C57BL6 mice infected with Helicobacter pylori SS1 strain. Helicobacter 8(4): 412, 2003

Compound(s) secreted by Lactobacillus casei strain Shirota YIT9029 irreversibly and reversibly impair the swimming motility of Helicobacter pylori and Salmonella enterica serovar Typhimurium, respectively. Microbiology 159(Pt 9): 1956-1971, 2013

Inhibition of Helicobacter pylori by Lactobacillus casei in vitro and in man. Gastroenterology 120(5 Suppl. 1): A 589-A 590, 2001

Release studies of Lactobacillus casei strain Shirota from chitosan-coated alginate-starch microcapsules in ex vivo porcine gastrointestinal contents. Letters in Applied Microbiology 41(6): 493-497, 2005

Lactobacillus casei Strain Shirota Enhances the In Vitro Antiproliferative Effect of Geniposide in Human Oral Squamous Carcinoma HSC-3 Cells. Molecules 23(5):, 2018

Immunomodulation by treatment with Lactobacillus casei strain Shirota. International Journal of Food Microbiology 41(2): 133-140, 1998

Properties of Lactobacillus casei Shirota strain as probiotics. Asia Pacific Journal of Clinical Nutrition 5(1): 29-30, 1996

Inhibition of Helicobacter pylori growth by Lactobacillus casei in fermented milk. Milchwissenschaft 57(9/10): 506-509, 2002

Inhibition of Helicobacter pylori by dairy starter bacteria Lactobacillus casei. Journal of Dairy Science 79(Suppl. 1): 122, 1996

In vitro antagonistic activity of Lactobacillus casei against Helicobacter pylori. Brazilian Journal of Microbiology 46(4): 1201-1206, 2016

Identification and quantification of Lactobacillus casei strain Shirota in human feces with strain-specific primers derived from randomly amplified polymorphic DNA. International Journal of Food Microbiology 126(1-2): 210-215, 2008

Health properties of milk fermented with Lactobacillus casei strain Shirota (LcS). Handbook of fermented functional foods: 145-175, 2003

Survival of Lactobacillus casei strain Shirota in the intestines of healthy Chinese adults. Microbiology and Immunology 59(5): 268-276, 2015

Transition of the probiotic bacteria, Lactobacillus casei strain Shirota, in the gastrointestinal tract of a pig. International Journal of Food Microbiology 96(1): 61-66, 2004