In vitro growth of Tagetes patula L. hairy roots, production of thiophenes and its mosquito larvicidal activity

Rajasekaran, T.; Ravishankar, G.A.; Reddy, B.O.

Indian Journal of Biotechnology 3(1): 92-96

2004


ISSN/ISBN: 0972-5849
Accession: 004198143

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Abstract
Hairy root culture of T. patula was studied for thiophene production. Biomass production was evaluated based on fresh and dry weights. The analysis of thiophene was carried out by flame ionization detection (FID) and mass spectral analysis (GC-MS). The separation profile of the thiophenes indicated the presence of several structurally different thiophenes, predominantly alpha -terthienyl, which was confirmed by FID and GC-MS analysis. The maximum accumulation of biomass (0.27 g dry weight per culture) was recorded on the 12th day. The thiophene content was highest (0.064%) on the 9th day. Thiophene produced in hairy roots of T. patula showed larvicidal effect against mosquito (Culex quinquefasciatus) larvae.