+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography



Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography



Journal of Chromatography, A 1063(1/2): 161-169



Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1:1, v:v). By injecting 200 mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165 mg of acteoside and 17.5 mg of isoacteoside from 978 mg extract. The recovery rates for acteoside and isoacteoside were 90 and 84%, respectively, and the purities were 98 and 94%, respectively. The HSCCC fractions were analyzed by HPLC and the structures were identified by UV, LC-APCI-MS in negative ion mode, and confirmed by NMR experiments.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004215544

Download citation: RISBibTeXText

PMID: 15700468

DOI: 10.1016/j.chroma.2004.11.024


Related references

Preparative isolation and purification of acteoside and 2'-acetyl acteoside from Cistanches salsa (C.A. Mey.) G. Beck by high-speed counter-current chromatography. Journal of Chromatography, A 912(1): 181-185, 2001

Isolation and purification of flavones from Murraya exotica L. by high-speed counter-current chromatography. Se Pu 28(4): 383-387, 2012

Isolation and purification of inflacoumarin A and licochalcone A from licorice by high-speed counter-current chromatography. Journal of Chromatography, A 1048(1): 51-57, 2004

Isolation and purification of seven lignans from Magnolia sprengeri by high-speed counter-current chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 879(31): 3775-3779, 2012

Isolation and purification of prenylated phenolics from Amorpha fruticosa by high-speed counter-current chromatography. Journal of Separation Science 38(16): 2924-2929, 2016

Isolation and purification of phenylethanoid glycosides from Cistanche deserticola by high-speed counter-current chromatography. Food Chemistry 108(2): 702-710, 2008

Preparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatography. Journal of Chromatography. A 925(1-2): 133-137, 2001

Preparative isolation and purification of four compounds from Cistanches deserticola Y.C. Ma by high-speed counter-current chromatography. Molecules 17(7): 8276-8284, 2012

Isolation and purification of coumarin compounds from Cortex fraxinus by high-speed counter-current chromatography. Journal of Chromatography. A 1072(2): 195-199, 2005

Isolation and purification of canthaxanthin from the microalga Chlorella zofingiensis by high-speed counter-current chromatography. Journal of Separation Science 29(5): 699-703, 2006

Isolation and purification of clemastanin B and indigoticoside A from Radix Isatidis by high-speed counter-current chromatography. Journal of Chromatography. A 1091(1-2): 89-93, 2006

Isolation and purification of flavonoid glucosides from Radix Astragali by high-speed counter-current chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 877(8-9): 697-702, 2009

Isolation and purification of clemastanin B and indigoticoside A from Radix isatidis by high-speed counter-current chromatography. Journal of Chromatography, A 1091(1/2): 89-93, 2005

Isolation and purification of oridonin from the whole plant of Isodon rubescens by high-speed counter-current chromatography. Molecules 16(9): 7949-7957, 2012

Preparative isolation and purification of capsaicinoids from Capsicum frutescens using high-speed counter-current chromatography. Separation and Purification Technology 64(3): 304-308, 2009