+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Molecular cloning and characterization of an almond 9-hydroperoxide lyase, a new CYP74 targeted to lipid bodies



Molecular cloning and characterization of an almond 9-hydroperoxide lyase, a new CYP74 targeted to lipid bodies



Journal of Experimental Botany 56(419): 2321-2333



Oxylipin metabolism represents one of many defence mechanisms employed by plants. It begins with the oxygenation of polyunsaturated fatty acids by lipoxygenases to form fatty acid hydroperoxides that are substrates for several enzymes, including specialized cytochrome P450s known as CYP74s. The targeting of a new CYP74, a 9-hydroperoxide lyase (HPL) from almonds, to the endomembrane system and lipid bodies, both as enzyme activity in almond seeds and as GFP fusions transiently expressed in tobacco protoplasts, is described. Such association of a CYP74 with lipid bodies has not been reported previously. Also described are the properties of a 9-HPL gene, the developmental regulation of its expression, the production and characterization of recombinant 9-HPL in Escherichia coli, and the developmental correlation between gene expression, enzyme activity, and the appearance of volatile C9 aldehydes from HPL action.

(PDF emailed within 0-6 h: $19.90)

Accession: 004239178

Download citation: RISBibTeXText

PMID: 16014368

DOI: 10.1093/jxb/eri225


Related references

Molecular cloning, expression, and enzymatic characterization of Solanum tuberosum hydroperoxide lyase. European Food Research and Technology 234(4): 723-731, 2012

Molecular Cloning and Characterization of Hydroperoxide Lyase Gene in the Leaves of Tea Plant (Camellia sinensis). Journal of Agricultural and Food Chemistry 64(8): 1770-1776, 2016

Determinants governing the CYP74 catalysis: conversion of allene oxide synthase into hydroperoxide lyase by site-directed mutagenesis. Febs Letters 582(23-24): 3423-3428, 2008

Cloning and characterization of a cDNA encoding hydroperoxide lyase in tomato. Plant Biology (Rockville) : 77, 1999

Molecular cloning and expression of Arabidopsis fatty acid hydroperoxide lyase. Plant & Cell Physiology 40(5): 477-481, 1999

Purification and molecular cloning of bell pepper fruit fatty acid hydroperoxide lyase. Physiology biochemistry and molecular biology of plant lipids 350, 1997

On the specificity of lipid hydroperoxide fragmentation by fatty acid hydroperoxide lyase from Arabidopsis thaliana. Journal of Plant Physiology 160(7): 803-809, 2003

Purification, molecular cloning, and expression of the gene encoding fatty acid 13-hydroperoxide lyase from guava fruit (Psidium guajava). Lipids 35(7): 709-720, 2000

Molecular characterization of an Arabidopsis gene encoding hydroperoxide lyase, a cytochrome P-450 that is wound inducible. Plant Physiology 117(4): 1393-1400, 1998

Formation of C6-aldehydes in Arabidopsis Isolation of mutants deficient in their formation and molecular cloning of fatty acid hydroperoxide lyase. Plant Biology (Rockville) : 65, 1998

Biogenesis of volatile aldehydes from fatty acid hydroperoxides: molecular cloning of a hydroperoxide lyase (CYP74C) with specificity for both the 9- and 13-hydroperoxides of linoleic and linolenic acids. Archives of Biochemistry and Biophysics 386(2): 281-289, 2001

Molecular cloning of a divinyl ether synthase. Identification as a CYP74 cytochrome P-450. Journal of Biological Chemistry 276(5): 3620-3627, 2000

Polysaccharide lyase: molecular cloning of gellan lyase gene and formation of the lyase from a huge precursor protein in Bacillus sp. GL1. Archives of Biochemistry and Biophysics 354(1): 31-39, 1998

Cloning and secretion of tomato hydroperoxide lyase in Pichia pastoris. Process Biochemistry 40(1): 95-102, 2005

Molecular cloning and biochemical characterization of a lipoxygenase in almond (Prunus dulcis) seed. European Journal of Biochemistry 268(5): 1500-1507, 2001