+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Molecular phylogeny of isolates of Ctenocephalides felis and related species based on analysis of ITS1, ITS2 and mitochondrial 16S rDNA sequences and random binding primers

Molecular phylogeny of isolates of Ctenocephalides felis and related species based on analysis of ITS1, ITS2 and mitochondrial 16S rDNA sequences and random binding primers

Parasitology Research 94(3): 219-226

The phylogenetic relationships among 31 different flea isolates representing seven different species were studied by nucleotide sequence comparison of the internal transcribed spacer 1 (ITS1), internal transcribed spacer 2 (ITS2) and/or mitochondrial 16S ribosomal RNA gene (mt16S-rDNA) to examine the patterns of variation. Results show that all regions are useful in discriminating among flea species. In Ctenocephalides felis and Tunga penetrans, some differences in these gene regions occurred among different isolates within the same species. In the latter case, the differences are in the mt16S-rDNA region, with one isolate showing 48% divergence in nucleotide sequence. The taxonomic implications of this result are unclear at present. The gene regions revealed differences between C. felis isolates only after DNA sequencing the PCR products. Further differentiation among C. felis isolates was obtained using four different random binding primers (decamers) and primers for mammalian aldolase to amplify narrow differences in the genome. Using these primers we were able to discriminate between different C. felis isolates and determine that some of the genetic variation coincided with minor differences in response to the control agent imidacloprid. However, overall findings do not support the existence of subspecies of C. felis.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 004240218

Download citation: RISBibTeXText

PMID: 15340839

DOI: 10.1007/s00436-004-1201-x

Related references

Sequencing for complete rDNA sequences (18S, ITS1, 5.8S, ITS2, and 28S rDNA) of Demodex and phylogenetic analysis of Acari based on 18S and 28S rDNA. Parasitology Research 111(5): 2109-2114, 2012

Molecular identification of Anselmella miltoni Girault through analysis of rDNA ITS1 and ITS2 sequences. Chinese Journal of Applied Entomology 49(2): 448-453, 2012

Molecular phylogenetic relationships in Aveneae (Poaceae) species and other grasses as inferred from ITS1 and ITS2 rDNA sequences. Plant Systematics and Evolution 213(3/4): 233-250, 1998

Phylogenetic analysis of the lichen family Umbilicariaceae based on nuclear ITS1 and ITS2 rDNA sequences. Lichenologist 31(5): 477-489, 1999

Molecular Identification and Differentiation of Fasciola Isolates Using PCR- RFLP Method Based on Internal Transcribed Spacer (ITS1, 5.8S rDNA, ITS2). Iranian Journal of Parasitology 6(3): 35-42, 2011

The placement of South African strains of Beauveria in a phylogeny inferred from rDNA ITS1-5.8S-ITS2 sequences. Journal of General and Applied Microbiology 57(5): 269-276, 2011

Molecular phylogeny of Pseudokeronopsis Protozoa, Ciliophora, Urostylida, with reconsideration of three closely related species at inter- and intra-specific levels inferred from the small subunit; ribosomal RNA gene and the ITS1-58S-ITS2 region sequences. Journal of Zoology (London) 275(3): 268-275, 2008

Molecular identification of Nilaparvata lugens Stal, Sogatella furcifera Horvath and Laodelphax striatellus Fallen Homoptera Delphacidae based on rDNA ITS1 and ITS2 sequences. Acta Entomologica Sinica 52.11 (November 20): 1266-1272, 2009

The taxonomic position of Saccharomyces boulardii as evaluated by sequence analysis of the D1/D2 domain of 26S rDNA, the ITS1-5.8S rDNA-ITS2 region and the mitochondrial cytochrome-c oxidase II gene. Systematic and Applied Microbiology 26(4): 564-571, 2003

Phylogenetic analysis of selected genera of Pezizales, inferred from 5.8S rDNA, ITS1 and ITS2 sequences. Systema Ascomycetum 13(1): 1-12, 1994

A phylogenetic perspective on larval spine morphology in Leucorrhinia (Odonata: Libellulidae) based on ITS1, 5.8S, and ITS2 rDNA sequences. Molecular Phylogenetics and Evolution 30(3): 653-662, 2004

Phylogenetic analysis of Phytophthora species based on ITS1 and ITS2 sequences of the ribosomal RNA gene repeat. Mycological Research 101(6): 667-677, 1997

Molecular phylogeny of Giardia lamblia based on ITS1-5.8 Sr-RNA-ITS2 sequence. Acta Zoologica Sinica 52(5): 954-958, 2006

Phylogenetic analysis of the ITS1, 58S and ITS2 rDNA sequences of Lacazia loboi confirms the placement of this unique fungal pathogen within the Onygenales. Abstracts of the General Meeting of the American Society for Microbiology 101: 381, 2001

A molecular phylogeny of the marine mussel genus Perna (Bivalvia: Mytilidae) based on nuclear (ITS1&2) and mitochondrial (COI) DNA sequences. Molecular Phylogenetics and Evolution 44(2): 685-698, 2007