Novel inhalational murine model of invasive pulmonary aspergillosis
Sheppard, D.C.; Rieg, G.; Chiang, L.Y.; Filler, S.G.; Edwards, J.E.; Ibrahim, A.S.
Antimicrobial Agents and ChemoTherapy 48(5): 1908-1911
ISSN/ISBN: 0066-4804 PMID: 15105158 DOI: 10.1128/aac.48.5.1908-1911.2004
We developed a novel model of invasive aspergillosis (IA) that recapitulates human disease. Mice were immunosuppressed with cyclophosphamide and cortisone acetate and then infected in an aerosol chamber. This procedure reproducibly delivered 1x103 to 3x103 conidia to the lungs. Lethal pulmonary IA developed over 2 weeks and was prevented by amphotericin B.