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Nucleotide sequence analysis of nucleocapsid protein gene of canine distemper virus isolates in Thailand



Nucleotide sequence analysis of nucleocapsid protein gene of canine distemper virus isolates in Thailand



Veterinary Microbiology 105(2): 137-142



The C-terminal part of the nucleocapsid protein gene of 13 canine distemper virus (CDV) isolates from Thailand, were analyzed. The nucleotide sequences were assigned to two clusters; cluster A exhibited a high degree of homology with the vaccine strain Onderstepoort, 99.10 and 97.61%, respectively, in the two isolates examined. Cluster B appeared closely related to virulent strains registered in the GeneBank database and to the virulent reference strain (A75/17); a total of 11 samples were analyzed, with 94.63-99.10% homology at the same position. The deduced amino acid sequences correlated with the two-nucleotide sequence clusters. However, there was no association among the CDV groups with histories of vaccination, sex, ages, clinical findings and evidence of viral antigen in tissues.

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Accession: 004251511

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PMID: 15627525

DOI: 10.1016/j.vetmic.2004.10.011


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