EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Optimization of the detection method for okadaic acid using liquid chromatography with high efficiency in shellfish


Optimization of the detection method for okadaic acid using liquid chromatography with high efficiency in shellfish



Higiene Alimentar 17(114/115): 29-33



The objective of this study was to optimize the HPLC-FD (fluorescence detection) method used for detecting okadaic acid in shellfish. The substitution of N,N-diisopropylethylamine by pyridine, ammonium hydroxide or triethylamine for the derivatization of okadaic acid with 1-(bromoacetyl) pyrene was tested. These possible substitutes were used as either pure or diluted with acetonitrile at different concentrations, with different reaction times at 75+or-1 degrees C.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 004257503

Download citation: RISBibTeXText

Related references

Development and validation of a high-performance liquid chromatographic method using fluorimetric detection for the determination of the diarrhetic shellfish poisoning toxin okadaic acid without chlorinated solvents. Journal of Chromatography A 876(1-2): 117-125, 2000

Identification of 19-epi-okadaic acid, a new diarrhetic shellfish poisoning toxin, by liquid chromatography with mass spectrometry detection. Marine Drugs 6(3): 489-495, 2008

Use of triethylamine in the derivatization of okadaic acid detected by high performance liquid chromatography on Perna perna shellfish collected from Sepetiba Bay. Revista Brasileira de Medicina Veterinaria 25(4): 158-162, 2003

Diarrhetic shellfish toxins in mussels: Optimization of HPLC method for okadaic acid determination. Rivista di Scienza dell'Alimentazione 22(4): 443-454, 1993

Improvement of the efficiency and simplification of ELISA tests for rapid and ultrasensitive detection of okadaic acid in shellfish. Food Control 30(1): 144-149, 2013

A Rapid Method for the Detection of Diarrhetic Shellfish Toxins and Azaspiracid Shellfish Toxins in Washington State Shellfish by Liquid Chromatography Tandem Mass Spectrometry. Journal of Aoac International 103(3): 792-799, 2020

Simultaneous determination of okadaic acid, dinophysistoxin, pectenotoxin and yessotoxin in shellfish by liquid chromatography-tandem mass spectrometry. Se Pu 30(3): 256-261, 2012

Immunoaffinity extraction as a new approach for an improved liquid chromatography-mass spectrometric or fluorimetric determination of okadaic acid in shellfish and algae. Analytica Chimica Acta 407(1-2): 173-186, 2000

Selective enrichment and quantification of okadaic acid in shellfish using an immunomagnetic-bead-based liquid chromatography with tandem mass spectrometry assay. Journal of Separation Science 42(7): 1423-1431, 2019

Selective analysis of the okadaic acid group in shellfish samples using fluorous derivatization coupled with liquid chromatography-tandem mass spectrometry. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 1173: 122681, 2021

Liquid chromatography-electrospray ionization mass spectrometry of the diarrhetic shellfish-poisoning toxins okadaic acid, dinophysistoxin-1 and pectenotoxin-6 in bivalves. Journal of Chromatography A 874(2): 199-206, 2000

Development of a novel immunobiosensor method for the rapid detection of okadaic acid contamination in shellfish extracts. Analytical and Bioanalytical Chemistry 389(2): 581-587, 2007

Liquid chromatography-linked protein phosphate bioassay : a highly sensitive marine bioscreen for okadaic acid and related diarrhetic shellfish toxins. Toxicon (Oxford) 29(4-5): 469-477, 1991

Strategies for the elimination of matrix effects in the liquid chromatography tandem mass spectrometry analysis of the lipophilic toxins okadaic acid and azaspiracid-1 in molluscan shellfish. Journal of Chromatography A 1217(45): 7123-7130, 2010

A routine HPLC fluorescence method for the determination of the diarrhetic shellfish toxins okadaic acid and DTX-1 in shellfish. Canadian Technical Report of Fisheries and Aquatic Sciences 1995(1985): I-VII, 1-26, 1995