Plant regeneration of Withania somnifera (L.) Dunal (Ashwagandha) from nodal segments derived callus an endangered medicinal plant in Bangladesh
Siddique, N.A.; Bari, M.A.; Sharmin Shahnewaz; Rahman, M.H.; Hasan, M.R.; Khan, M.S.I.; Islam, M.S.
Journal of Biological Sciences 4(2): 219-223
2004
ISSN/ISBN: 0021-9282 Accession: 004272352
Nodal segments of W. somnifera were cultured in MS medium supplemented with different plant growth regulators. Callus formation was induced in MS medium supplemented with 2,4-D, benzyladenine (BA) and kinetin, with MS medium containing 1 mg BA/litre and 2 mg kinetin/litre inducing the highest callus formation. Adventitious root developed when the calluses were transferred to MS medium supplemented with BA and kinetin.