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Preparation and characterization of monoclonal antibodies against SARS-associated coronavirus nucleocapsid protein



Preparation and characterization of monoclonal antibodies against SARS-associated coronavirus nucleocapsid protein



Chinese Journal of Experimental and Clinical Virology 18(4): 316-320



A study was conducted to obtain monoclonal antibodies against severe acute respiratory syndrome (SARS)-associated Coronavirus (SARS-CoV) nucleocapsid protein in order to develop a diagnostic test for SARS and study the pathogenesis of the disease. BALB/c mice were immunized with purified N protein of SARS-CoV. Hybridoma cell lines secreting monoclonal antibodies against SARS-CoV nucleocapsid were established after cell fusion with mouse splenic cells and SP2/0 cells.

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