Preparation and purification of Epigallocatechin by high-speed countercurrent chromatography (HSCCC)

Cao XueLi; Ito, Y.

Journal of Liquid Chromatography and Related Technologies 27(1): 145-152


ISSN/ISBN: 1082-6076
DOI: 10.1081/jlc-120027091
Accession: 004279908

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Epigallocatechin (EGC) was prepared by the degallation of epigallocatechin-O-3-gallate (EGCG). EGCG was completely converted to EGC and gallic acid by adding 4 mg tannase/100 mg EGCG at the concentration of 2 mg/ml under pH 6.0, at 35 degrees C for 30 minutes. EGC was then separated from the reaction mixture by HSCCC, using a two-phase solvent system composed of hexane-ethyl acetate-water (1:9:10, v/v/v). Finally, 1.3 g of EGC at 97% purity was prepared from about 2.3 g of EGCG at 85% purity, indicating that EGC was almost completely recovered by HSCCC.