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Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid state fermentation

Uyar, F.; Baysal, Z.

Process Biochemistry 39(12): 1893-1898

2004


ISSN/ISBN: 1359-5113
DOI: 10.1016/j.procbio.2003.09.016
Accession: 004283560

Production of alkaline protease employing the laboratory isolate, Bacillus sp. under solid state fermentation (SSF) was optimized. The effect of wheat bran and lentil husk was examined. Wheat bran showed highest enzyme production. The appropriate incubation time, inoculum size, moisture level and buffer solution level were determined. Maximum yields of 429.041 and 168.640 U g-1 were achieved by employing wheat bran and lentil husk as substrates in 0.1 M carbonate/bicarbonate buffer at pH 10 with 30 and 40% initial moisture level at 24 h. Inoculum size and buffer solution level were found to be 20 and 25% and 0.5:1 for wheat bran and lentil husk, respectively.

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