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RFLP analysis of ITS region of rDNA in the falcate-spored species of Colletotrichum

RFLP analysis of ITS region of rDNA in the falcate-spored species of Colletotrichum

Acta Phytopathologica Sinica 34(5): 431-436

The genetic divergence among 38 strains belonging to the falcate-spored species of Colletotrichum was assessed by RFLP analysis based on the rDNA ITS region. The PCR-amplified ITS region (ITS4-ITS5) was ~650 bp in length in all the tested strains. RFLP patterns of ITS products digested with different endonucleases (Alu I, BsuR I, Hin6 I, Hpa II and Taq I) were not distinguishable within same species, but clearly were different at the interspecies level.

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