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Rapid analysis of legume root nodule development using confocal microscopy



Rapid analysis of legume root nodule development using confocal microscopy



New Phytologist 163(3): 661-668



A rapid method for detailed analysis of nodule formation has been developed. Inoculated root tissues of Medicago truncatula and Pisum sativum Early Alaska were stained with SYTO 13, a cell-permeant fluorescent nucleic acid-binding dye, and visualized using confocal laser scanning microscopy (CLSM). Structures with high concentrations of DNA and RNA, such as plant cell nuclei and bacteria, labelled strongly.

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Accession: 004292310

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DOI: 10.1111/j.1469-8137.2004.01138.x


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