Research and comparative study of proteasic activities of neutral castes of termite Macrotermes subhyalinus (Termitidae, Macrotermitinae) and its symbiotic fungus Termitomyces sp

Kouame, L.P.; Yapo, P.A.; Niamke, S.L.; Kamenan, A.

Tropicultura 22(3): 151-155


ISSN/ISBN: 0771-3312
Accession: 004300998

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Crude extracts from neutral castes of the termite M. subhyalinus, its symbiotic fungus Termitomyces sp. and its comb were not able to hydrolyse acyl-pNA evaluated on acid medium. However, these substrates on alkaline medium were hydrolysed, indicating that these enzymatic sources possess proteolytic activities. The hydrolysed acyl-pNA were gly-pNA, leu-pNA, met-pNA, phe-pNA, pro-pNA, lys-pNA, arg-pNA and asp-pNA. The highest proteolytic activities were obtained with leu-pNA and BA-pNA with a M. subhyalinus worker. It was, therefore, more active than the soldier. Proteolytic activities were higher in small soldiers than in large ones. The proteolytic activities of the fungus were higher than those of the fungus comb, its growth backbone. Mutual competition studies among the substrates best hydrolysed by the symbiotic fungus and worker showed that trypsic activities (arg-pNA, lys-pNA and BA-pNA) were derived from the same enzyme. This enzymatic reaction was also observed with leu-pNA, met-pNA and ala-pNA.