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Reverse transcriptase PCR diagnostic assay for the coronavirus associated with severe acute respiratory syndrome



Reverse transcriptase PCR diagnostic assay for the coronavirus associated with severe acute respiratory syndrome



Journal of Clinical Microbiology 42(5): 1994-1999



Recent outbreaks of severe acute respiratory syndrome (SARS) have spurred intense research efforts around the world to deal with the serious threat to health posed by this novel coronavirus. A rapid, reliable diagnostic assay is needed for monitoring the spread of the disease. Here we report a method for eliminating false-negative results and increasing test sensitivity, based on the hypothesis that the message encoded by the nucleocapsid (N) gene is the most abundant during viral infection.

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Accession: 004304687

Download citation: RISBibTeXText

PMID: 15131160

DOI: 10.1128/jcm.42.5.1994-1999.2004


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