+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Role of oxidative stress in neurodegeneration: recent developments in assay methods for oxidative stress and nutraceutical antioxidants

Role of oxidative stress in neurodegeneration: recent developments in assay methods for oxidative stress and nutraceutical antioxidants

Progress in Neuro-Psychopharmacology and Biological Psychiatry 28(5): 771-799

Reactive oxygen species (ROS) are produced in the course of normal metabolism and they serve important physiological functions. However, because of their high reactivity, accumulation of ROS beyond the immediate needs of the cell may affect cellular structure and functional integrity, by bringing about oxidative degradation of critical molecules, such as the DNA, proteins, and lipids. Although cells possess an intricate network of defense mechanisms to neutralize excess ROS and reduce oxidative stress, some tissues, especially the brain, are much more vulnerable to oxidative stress because of their elevated consumption of oxygen and the consequent generation of large amounts of ROS. For the same reason, the mitochondrial DNA (mtDNA) of brain cells is highly susceptible to structural alterations resulting in mitochondrial dysfunction. Several lines of evidence strongly suggest that these effects of ROS may be etiologically related to a number of neurodegenerative disorders. Nutraceutical antioxidants are dietary supplements that can exert positive pharmacological effects on specific human diseases by neutralizing the negative effects of ROS. The present communication concentrates on a review of recent concepts and methodological developments, some of them based on the results of work from our own laboratory, on the following aspects: (1) the complex interactions and complementary interrelationships between oxidative stress, mitochondrial dysfunction, and various forms of neural degeneration; (2) fractionation and isolation of substances with antioxidant properties from plant materials, which are extensively used in the human diet and, therefore, can be expected to be less toxic in any pharmacological intervention; (3) recent developments in methodologies that can be used for the assay of oxidative stress and determination of biological activities of exogenous and endogenous antioxidants; and (4) presentation of simple procedures based on polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) of the resulting amplicon for investigations of structural alterations in mtDNA.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 004307197

Download citation: RISBibTeXText

PMID: 15363603

DOI: 10.1016/j.pnpbp.2004.05.023

Related references

Role of oxidative stress in pathology of chronic prostatitis/chronic pelvic pain syndrome and male infertility and antioxidants function in ameliorating oxidative stress. Biomedicine and PharmacoTherapy 106: 714-723, 2018

Toxic metals and oxidative stress Part II: Role of antioxidants in metal-induced oxidative damage. Current Topics in Toxicology, Volume 1: 53-71, 2004

Comparative analysis of different dietary antioxidants on oxidative stress pathway genes in L6 myotubes under oxidative stress. Cytotechnology 70(4): 1177-1192, 2018

Oxidative renal cell injury induced by calcium oxalate crystal and renoprotection with antioxidants: a possible role of oxidative stress in nephrolithiasis. Journal of Endourology 24(3): 339-345, 2010

Biomarkers of oxidative stress: methods and measures of oxidative DNA damage (COMET assay) and telomere shortening. Methods in Molecular Biology 610: 245-261, 2010

Oxidative stress, antioxidants, and assessment of oxidative stress in dogs and cats. Journal of the American Veterinary Medical Association 231(5): 714-720, 2007

Stress and brain oxidative damage The role of monoamine transmitters, stress-related hormones, and antioxidants. Neuropsychopharmacology 23(S2): S53, 2000

High temperature-induced oxidative stress in Lens culinaris , role of antioxidants and amelioration of stress by chemical pre-treatments. Journal of Plant Interactions 6(1): 43-52, 2011

Quantifying cellular oxidative stress by dichlorofluorescein assay and protection against oxidative stress in PC 12 cells by some dietary flavonoids. FASEB Journal 12(5): A828, March 20, 1998

Biomarkers of oxidative stress study VI. Endogenous plasma antioxidants fail as useful biomarkers of endotoxin-induced oxidative stress. Free Radical Biology and Medicine 81: 100-106, 2015

Dual Effects of Antioxidants in Neurodegeneration: Direct Neuroprotection against Oxidative Stress and Indirect Protection via Suppression of Gliamediated Inflammation. Current Pharmaceutical Design 12(27): 3521-3533, 2006

Dual effects of antioxidants in neurodegeneration: direct neuroprotection against oxidative stress and indirect protection via suppression of glia-mediated inflammation. Current Pharmaceutical Design 12(27): 3521-3533, 2006

Oxidative stress in hemodialysis patients receiving intravenous iron therapy and the role of N-acetylcysteine in preventing oxidative stress. Saudi Journal of Kidney Diseases and Transplantation 21(5): 852-858, 2010

Putative role of intracellular Zn(2+) release during oxidative stress: a trigger to restore cellular thiol content that is decreased by oxidative stress. Journal of Physiological Sciences 61(5): 403-409, 2012

The role of oxidative stress in zearalenone-mediated toxicity in Hep G2 cells: oxidative DNA damage, gluthatione depletion and stress proteins induction. Toxicology 232(3): 294-302, 2007