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Schistosoma japonicum recombinant ferritin: expression, purification and its protective immunity against Schistosoma japonicum in mice


Schistosoma japonicum recombinant ferritin: expression, purification and its protective immunity against Schistosoma japonicum in mice



Chinese Journal of Zoonoses 20(2): 113-116



The objectives of this study were to express and purify the recombinant S. japonicum ferritin (rsjferritin), and to explore the protective immunity against S. japonicum induced by the protein in mice. The ferritin gene was subcloned into the expression vector pGMC. The rsjferritin was expressed at a high level in E. coli 2566 by IPTG, and was analysed using electrophoretic chromatography, SDS-PAGE and western blotting.

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