Somatic embryogenesis in Withania somnifera (L.) Dunal
Gita Rani; Virk, G.S.; Avinash Nagpal
Journal of Plant Biotechnology 6(2): 113-118
Somatic embryos were formed from calluses obtained from axillary shoots (raised from nodal segments of glasshouse-grown plants under aseptic conditions), internodal segments (from in vitro-raised plants), and root and cotyledonary leaf segments (from in vitro-raised seedlings) after 8 weeks of initial culture. Embryo formation was the highest (97.33%) from cotyledonary leaf callus on Murashige and Skoog's (MS) medium containing kinetin (KN) (3 mg/L).