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Standardization of crude extract of neem seed kernels (Azadirachta indica A. Juss) and commercial neem based formulations using HPTLC and extended length packed-columns SFC method



Standardization of crude extract of neem seed kernels (Azadirachta indica A. Juss) and commercial neem based formulations using HPTLC and extended length packed-columns SFC method



Chromatographia 62(3/4): 183-195



Two chromatographic techniques are described for the separation and quantitative determination of azadirachtin A and B, salannin, and nimbin present in the crude extract of neem seed kernels and commercial neem based formulations. The high performance thin-layer chromatography (HPTLC) separation of markers was carried out on Merck TLC aluminium sheets of silica gel 60 F254 using ethylacetate-benzene (7.0: 3.0, v/v) as mobile phase.

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Accession: 004327271

Download citation: RISBibTeXText

DOI: 10.1365/s10337-005-0588-6


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