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The effect of prolactin and bovine follicular fluid on the in vitro maturation and subsequent development of immature buffalo (Bubalus bubalis) oocytes

Sesay, A.R.; Shi DeShun

Journal of South China Agricultural University 25(2): 93-97

2004


ISSN/ISBN: 1001-411X
Accession: 004350559

Buffalo oocytes with compact cumulus cells were collected from slaughterhouse ovaries and cultured in TCM 199 supplemented with 5% oestrus cow serum (ECS) + 0.1 micro g/ml FSH and prolactin (PRL) 0, 0.1, 1.0, 10 micro g/ml in Experiment 1, and with 0% bovine follicular fluid (BFF) + 1.0 micro g ml PRL, 5% BFF, 5% BFF + 1.0 micro g/ml PRL in Experiment 2 and incubated at 38.5 degrees C and in phi =5% CO2 humidified air.

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