+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Uterine immune reaction and reproductive performance of sows inseminated with extended semen and infused with pooled whole dead semen



Uterine immune reaction and reproductive performance of sows inseminated with extended semen and infused with pooled whole dead semen



Journal of Animal Science 81(11): 2818-2825



The objective of this study was to investigate the effect of infusing whole dead semen (WDS) after AI with diluted commercial semen on uterine inflammatory reaction and embryonic survival rate in gilts. Sixty Yorkshire-Landrace gilts were assigned at their second estrus to one of the following AI treatments: 1) commercial semen adjusted to 1 [times] 109 sperm cells (S1) per dose, followed by an infusion of 80 mL of WDS (S1-WDS); 2) S1 followed by an infusion of 80 mL of Beltsville Thawing Solution (S1-BTS); 3) commercial semen adjusted to 3 [times] 109 sperm cells (S3) per dose, followed by an infusion of 80 mL of BTS (S3-BTS); and 4) a negative control group, in which gilts received two infusions of 80 mL of BTS (BTS). Two days after the first AI, eight gilts from Groups 1, 2, and 4 were slaughtered and reproductive tracts were collected. One horn was cut open longitudinally along the antimesometrial aspect and endometrial samples were taken and immediately frozen for analysis of messenger RNA (mRNA) abundance for inflammatory cytokines and growth factors. The other horn was flushed with 20 mL of PBS, and the contents of interferon-c (IFN-c), tumor necrosis factor-a (TNF-a) and transforming growth factor-b1 (TGF-b1) were determined by ELISA. On d 25 after AI, gilts from Groups 1, 2, and 3 were slaughtered and their reproductive tracts were collected to evaluate the number of fetuses and corpora lutea. On d 2 after the first AI, only TGF-b1 was detected in the flush of all gilts, and no difference was observed between S1-WDS, S1-BTS, and BTS gilts. Endometrial levels of IFN-c and interleukin (IL)-6 mRNA were marked in all gilts, but they were not affected by the AI treatments, whereas the mRNA abundances for IL-1 and IL-2 were negligible. Infusions of WDS or BTS after a fertile AI did not affect IGF-I, IGF-I receptor, or IGF-II mRNA levels compared with gilts infused with BTS only, whereas the mRNA abundance for the IGF-II receptor was decreased (P < 0.05) in WDS-infused gilts. In gilts inseminated with S1 doses, infusion of WDS did not affect the number of live embryos. Although infusions of WDS did not affect the mRNA level and secretion of the cytokines measured and did not improve embryonic survival rates, further studies are needed to better understand the influence of semen composition on the uterine response after mating. Reprinted by permission of the publisher.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 004379690

Download citation: RISBibTeXText

PMID: 14601886


Related references

Uterine immune reaction and reproductive performance of sows inseminated with extended semen and infused with pooled whole dead semen1,2,3. Journal of Animal Science 81(11): 2818-2825, 2003

Achromobacter xylosoxidans in extended semen causes reproductive failure in artificially inseminated sows and gilts. Journal Of Swine Health And Production: 6, 316-322, 2008

Reproductive performance of sows inseminated with fresh or thawed semen. Agriculture and forestry bulletin Alberta University Faculty of Extension: (special issue) 43-44, 1985

Reproductive performance of sows inseminated once or twice using semen stored in kiew extender. Pesquisa Agropecuaria Brasileira 19(7): 909-914, 1984

Reproductive performance of sows inseminated with various doses of frozen-thawed semen. Journal of Dairy Science 93: 819-819, 2010

Prostaglandin F2alpha supplemented semen improves reproductive performance in artificially inseminated sows. Animal Reproduction Science 80(1-2): 113-120, 2004

Reproductive performance of sows inseminated with fresh or thawed semen influence of insemination at a fixed time or as indicated by changes in vaginal mucus conductivity. Canadian Journal of Animal Science 67(3): 865-868, 1987

Reproductive results in sows inseminated with the same semen diluted with 2 diluents. Rivista di Suinicoltura 33(9): 77-79, 1992

Effect of age of semen on fertilization rate and the number of accessory spermatozoa, with reference to the time of ovulation, in sows inseminated with fresh semen. Einfluss des Spermienalters auf Befruchtungsrate und akzessorische Spermienzahl unter berucksichtigung des Ovulationszeitpunktes bei der Frischspermabesamung des Schweines: 103 pp., 1991

Reproductive function of sows inseminated using diluted semen in Androhep EnduraGuard and Androbep Plus. Journal of Dairy Science 88: 70-70, 2005

Reproductive parameters of sows inseminated with semen diluted in coconut water after transcutaneous ultrasonography monitoring. Ciencia Animal Brasileira 4(1): 61-67, 2003

Reproductive performance and uterine prostaglandin secretion in gilts conditioned with dead semen and receiving dietary supplements of folic acid. Canadian Journal of Animal Science 80(3): 467-472, 2000

Reproductive performance of Kerry cattle inseminated with Charolais semen. Veterinary Record 92(1): 16-17, 1973

Dilution of porcine semen: role of seminal plasma on regulation of uterine immune response and reproductive performance in the sow. 34emes Journees de la Recherche Porcine, sous l' egide de l' Association Franccaise de Zootechnie, Paris, France, 5-7 fevrier 2002: 283-288, 2002

Reproductive performance of hens inseminated with fresh semen stored for up to 7 days. Monatshefte fur Veterinarmedizin 43(18): 646, 1988