Utilization of part of the region which codes for glycoprotein b in bovine herpesvirus 1.1, bovine herpesvirus 1.2 and bovine herpesvirus 5

Costa, E.A.; Barbosa Stancioli, E.F.; Leite, R.C.; Oliveira, G.D.R.; Rocha, M.A.

Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 57(2): 143-149

2005


ISSN/ISBN: 0102-0935
Accession: 004380001

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Abstract
A previously-developed polymerase chain reaction (PCR) was used for the amplification of part of the UL27 region of the bovine herpesvirus (BoHV)-1.1 genome, which codes for glycoprotein B, seeking the differentiation between BoHV-1.1 and BoHV-5 isolates. The PCR products generated from the BoHV-1 and BoHV-5 isolates showed a pattern of differentiated amplification of their molecular size. The PCR of the BoHV-5 products were sequenced and the results compared with the sequence of the BoHV-1.1 and BoHV-1.2 isolates previously deposited in the GenBank. It was verified that the difference between the PCR products is due to the greater number of repetitions of G-C present at the end of the gB codifier region. The most common repeat sequences were 5'-G(A/T)CC-3'. The analysis of these repetitions was shown to be an auxiliary tool in the differentiation between BoHV-1.1, BoHV-1.2 and BoHV-5 isolates.