+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

ydaG and ydbA of Lactococcus lactis encode a heterodimeric ATP-binding cassette-type multidrug transporter



ydaG and ydbA of Lactococcus lactis encode a heterodimeric ATP-binding cassette-type multidrug transporter



Journal of Biological Chemistry 279(33): 34449-34455



Multidrug resistance (MDR)-type transporters mediate the active extrusion of structurally and functionally dissimilar compounds from the cells, thereby rendering cells resistant to a range of drugs. The ydaG and ydbA genes of Lactococcus lactis encode two ATP-binding cassette half-transporters, which both share homology with MDR proteins such as LmrA from L. lactis or the mammalian P-glycoprotein. The ydaG/ydbA genes were cloned and expressed separately and jointly in L. lactis using the nisin-inducible system. When both proteins are co-expressed, several structurally dissimilar drugs such as ethidium, daunomycin, and BCECF-AM are extruded from the cell. YdaG and YdbA could be co-purified as a stable heterodimer. ATPase activity was found to be associated with the YdaG/YdbA heterodimer only and not with the individual subunits. Both the ydaG and ydbA genes are up-regulated in multidrug-resistant L. lactis strains selected for growth in the presence of a variety of toxic compounds. This is the first demonstration of a functional heterodimeric ATP-binding cassette-type MDR transporter.

(PDF emailed within 0-6 h: $19.90)

Accession: 004389335

Download citation: RISBibTeXText

PMID: 15192086

DOI: 10.1074/jbc.M404072200


Related references

Nucleotide-binding sites of the heterodimeric LmrCD ABC-multidrug transporter of Lactococcus lactis are asymmetric. Biochemistry 45(2): 648-656, 2006

Substrate specificity and ionic regulation of GlnPQ from Lactococcus lactis. An ATP-binding cassette transporter with four extracytoplasmic substrate-binding domains. Journal of Biological Chemistry 280(25): 23785-23790, 2005

An ABC-type multidrug transporter of Lactococcus lactis possesses an exceptionally broad substrate specificity. Drug Resistance Updates 3(6): 330-334, 2001

Energetics of wild-type and mutant multidrug resistance secondary transporter LmrP of Lactococcus lactis. Biochimica et Biophysica Acta 1658(3): 252-261, 2004

Gambogic acid as a non-competitive inhibitor of ATP-binding cassette transporter B1 reverses the multidrug resistance of human epithelial cancers by promoting ATP-binding cassette transporter B1 protein degradation. Basic & Clinical Pharmacology & Toxicology 112(1): 25-33, 2013

Backbone NMR resonance assignments of the nucleotide binding domain of the ABC multidrug transporter LmrA from Lactococcus lactis in its ADP-bound state. Biomolecular Nmr Assignments 6(1): 69-73, 2012

Photoaffinity labeling under non-energized conditions of a specific drug-binding site of the ABC multidrug transporter LmrA from Lactococcus lactis. Biochemical and Biophysical Research Communications 311(3): 696-701, 2003

The ABC-type multidrug resistance transporter LmrCD is responsible for an extrusion-based mechanism of bile acid resistance in Lactococcus lactis. Journal of Bacteriology 190(22): 7357-7366, 2008

Homology model of the multidrug transporter LmrA from Lactococcus lactis. Bioorganic & Medicinal Chemistry Letters 14(23): 5823-5826, 2004

LmrCD is a major multidrug resistance transporter in Lactococcus lactis. Molecular Microbiology 61(3): 771-781, 2006

Allosteric effects of ATP binding on the nucleotide-binding domain of a heterodimeric ATP-binding cassette transporter. Integrative Biology 8(11): 1158-1169, 2016

Characterization of the lacticin 481 operon: the Lactococcus lactis genes lctF, lctE, and lctG encode a putative ABC transporter involved in bacteriocin immunity. Applied and Environmental Microbiology 63(11): 4252-4260, 1997

LmrR is a transcriptional repressor of expression of the multidrug ABC transporter LmrCD in Lactococcus lactis. Journal of Bacteriology 190(2): 759-763, 2007