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Characterization of a cry4Ba-type gene of Bacillus thuringiensis israelensis and evidence of the synergistic larvicidal activity of its encoded protein with Cry2A delta -endotoxin of B. thuringiensis kurstaki on Culex pipiens (common house mosquito)



Characterization of a cry4Ba-type gene of Bacillus thuringiensis israelensis and evidence of the synergistic larvicidal activity of its encoded protein with Cry2A delta -endotoxin of B. thuringiensis kurstaki on Culex pipiens (common house mosquito)



Biotechnology and Applied Biochemistry 44(1): 19-25



A newly isolated strain of Bacillus thuringiensis, named BUPM97, was identified as affiliated to the israelensis subspecies. This strain was selected for its insecticidal activity against larvae of several dipteran insects, such as the common house mosquito (Culex pipiens). A 5 kb EcoRI fragment, containing a cry4Ba-type gene, named cry4BLB, was cloned from BUPM97. The sequencing of this gene revealed an open reading frame of 3411 bp encoding a protein of 1136 amino acid residues.

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Characterization of a cry4Ba-type gene of Bacillus thuringiensis israelensis and evidence of the synergistic larvicidal activity of its encoded protein with Cry2A delta-endotoxin of B. thuringiensis kurstaki on Culex pipiens (common house mosquito). Biotechnology and Applied Biochemistry 44(Pt 1): 19-25, 2006

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