+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Design of Shewanella-specific 16S rRNA primers and application to analysis of Shewanella in a minerotrophic wetland



Design of Shewanella-specific 16S rRNA primers and application to analysis of Shewanella in a minerotrophic wetland



Environmental Microbiology 8(3): 426-432



In this study, an existing probe was used as a polymerase chain reaction (PCR) primer to study iron-reducing members of the genus Shewanella in a minerotrophic wetland where iron reduction had previously been implicated. The probe was found to be non-specific and a new set of PCR primers were developed that were specific for Shewanella. These primers were used to analyse the wetland iron-reducing communities by characterizing 16S rRNA genes amplified from DNA extracted from peat. Polymerase chain reaction clone libraries were screened using restriction fragment length polymorphism and diagnostic operational taxonomic units for Shewanella species were identified. A statistical method was used to determine the coverage of the clone libraries, which was found to be between 83% and 97%. The dominant species in the wetland samples at two geochemically distinct zones were phylogenetically related to the iron-reducing microorganism Shewanella oneidensis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004413682

Download citation: RISBibTeXText

PMID: 16478449

DOI: 10.1111/j.1462-2920.2005.00908.x


Related references

Description of Shewanella glacialipiscicola sp. nov. and Shewanella algidipiscicola sp. nov., isolated from marine fish of the Danish Baltic Sea, and proposal that Shewanella affinis is a later heterotypic synonym of Shewanella colwelliana. International Journal of Systematic and Evolutionary Microbiology 57(Pt 2): 347-352, 2007

Differentiation of Shewanella putrefaciens and Shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16S rRNA gene sequence analysis. Applied and Environmental Microbiology 63(6): 2189-2199, 1997

Shewanella surugensis sp. nov., Shewanella kaireitica sp. nov. and Shewanella abyssi sp. nov., isolated from deep-sea sediments of Suruga Bay, Japan. International Journal of Systematic and Evolutionary Microbiology 56(Pt 7): 1607-1613, 2006

Phylogeny of marine and freshwater Shewanella: reclassification of Shewanella putrefaciens NCIMB 400 as Shewanella frigidimarina. International Journal of Systematic Bacteriology 49 Pt 1: 189-191, 1999

Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines. International Journal of Systematic and Evolutionary Microbiology 53(2): 491-499, 2003

Design and application of rRNA-targeted oligonucleotide probes for the dissimilatory iron- and manganese-reducing bacterium Shewanella putrefaciens. Applied and Environmental Microbiology 59(12): 4152-4160, 1993

Shewanella saliphila sp. nov., Shewanella ulleungensis sp. nov. and Shewanella litoralis sp. nov., isolated from coastal seawater. International Journal of Systematic and Evolutionary Microbiology 68(9): 2960-2966, 2018

Studies of iron-uptake mechanisms in two bacterial species of the shewanella genus adapted to middle-range (Shewanella putrefaciens) or antarctic (Shewanella gelidimarina) temperatures. Chemistry and Biodiversity 5(10): 2113-2123, 2008

Structural analysis of 3-isopropylmalate dehydrogenase from the obligate piezophile Shewanella benthica DB21MT-2 and the nonpiezophile Shewanella oneidensis MR-1. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 68(Pt 3): 265-268, 2012

Design of Vibrio 16S rRNA gene specific primers and their application in the analysis of seawater Vibrio community. Journal of Ocean University of China 5(2): 157-164, 2006

Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov. Archives of Microbiology 170(5): 331-338, 1998

Shewanella psychrophila sp. nov. and Shewanella piezotolerans sp. nov., isolated from west Pacific deep-sea sediment. International Journal of Systematic and Evolutionary Microbiology 57(Pt 1): 60-65, 2007

Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. International Journal of Systematic Bacteriology 48 Pt 1: 179-186, 1998

Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea. International Journal of Systematic and Evolutionary Microbiology 56(Pt 1): 243-249, 2006

Differentiation of Shewanella putrefaciens and Shewanella algae by polymerase chain reaction. Abstracts of the General Meeting of the American Society for Microbiology 98: 438-439, 1998