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Enhancement of in vitro maturation rate of oocytes in buffalo with a protein fraction obtained from the ovarian follicular fluid of buffalo

Gupta, P.S.P.; Ravindra, J.P.; Raghu, H.M.; Nandi, S.

Indian Journal of Animal Sciences 76(3): 208-213

2006


ISSN/ISBN: 0367-8318
Accession: 004426355

Ovarian follicular fluid is commonly being used as an input in the in vitro maturation (IVM) medium of oocytes. This study was conducted to find the oocyte stimulatory protein fractions of bubaline ovarian follicular fluid (buFF). A protein fraction in IVM medium of bubaline oocytes was analysed. A protein fraction precipitated from cell and steroid free buFF with ammonium sulfate at 0-25% saturation level was purified through sephadex-G 25 gel filtration column.

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