+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification and quantification of polyphenols in carob fruits (Ceratonia siliqua L.) and derived products by HPLC-UV-ESI/MSn

Identification and quantification of polyphenols in carob fruits (Ceratonia siliqua L.) and derived products by HPLC-UV-ESI/MSn

Journal of Agricultural and Food Chemistry 52(12): 3784-3791

The polyphenolic patterns of carob pods (Ceratonia siliqua L.) and derived products were identified and quantified using high-performance liquid chromatography-UV absorption-electrospray ion trap mass spectrometry after pressurized liquid extraction and solid-phase extraction. In carob fiber, 41 individual phenolic compounds could be identified. In addition, spectrophotometric quantification using the Folin-Ciocalteu and vanillin assays was performed, and the antioxidative activity was determined as the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Carob pods contain 448 mg/kg extractable polyphenols comprising gallic acid, hydrolyzable and condensed tannins, flavonol-glycosides, and traces of isoflavonoids. Among the products investigated, carob fiber, a carob pod preparation rich in insoluble dietary fiber (total polyphenol content = 4142 mg/kg), shows the highest concentrations in flavonol-glycosides and hydrolyzable tannins, whereas roasted carob products contain the highest levels of gallic acid. The production process seems to have an important influence on the polyphenolic patterns and quantities in carob products.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 004439007

Download citation: RISBibTeXText

PMID: 15186098

DOI: 10.1021/jf030660y

Related references

Tannins and polyphenols in carob pods (Ceratonia siliqua). J. Sci. Food Agric 14: 464-8. bibl. 16, 1963

Identification and characterization of Alternaria alternata (Fr.) Keissler causing Ceratonia Blight disease of carob (Ceratonia siliqua L.) in Turkey. European Journal of Plant Pathology 151(1): 73-86, 2018

Characterization of carob fruits (Ceratonia siliqua L.), cultivated in Spain for agroindustrial use. International Tree Crops Journal 9(1): 1-9, 1996

Mineral composition of pods and seeds of wild and grafted carob (Ceratonia siliqua L.) fruits. Scientia Horticulturae 167: 149-152, 2014

Determination of macronutrient contents in leaves, cuttings, fruits and seeds of carob (Ceratonia siliqua L.). Bahce 1998; 25(1/2): 61-69, 1996

Analysis of volatile components derived from the carob bean Ceratonia siliqua. Phytochemistry 31(89): 3113-3119, 1992

Isolation and identification of cyclitols in carob pods (Ceratonia siliqua L.). Journal of agricultural and food chemistry 34(5): 827-829, 1986

Isolation and identification of cyclitols in carob pods ceratonia siliqua. Journal of Agricultural & Food Chemistry 34(5): 827-829, 1986

The important potential ecological and economic value of carob (goat horn) Ceratonia siliqua L., the production of which is becoming secondary to citrus fruits in Kozan. Orman Muhendisligi 44(10/12): 38-40, 2005

Carob, ceratonia siliqua, a new dietetic. II. Antidiarrheal properties of carob meal and clinical use of carob bean meal for inspissation. Die Medizinische Welt 20(22): 747-749, 1951

Industrial extraction of the crystalline saccharide of the carob. 7. Identity of crystalline sugars from the carob (Ceratonia siliqua L. ) and from the Peruvian carob (Prosopis horrida, inermis pallida, etc. ) with cane and beet sugar. Ann. Chim. appl. Roma. 26: 89-95, 1936

Sensor analyses of volatile components derived from earth-almond Cyperus esculentus L. and carob Ceratonia siliqua L. Zeitschrift fuer Lebensmittel-Untersuchung und -Forschung A 208(5-6): 373-378, 1999

Diversity analysis of moroccan carob (Ceratonia siliqua L.) accessions using phenotypic traits and RAPD markers FT Analyse de la diversite des accessions du caroubier marocain (Ceratonia siliqua L.) utilisant des traits phenotypiques et des marqueurs RAPD. 2007

The occurrence of abscisic acid in inhibitors B1 and C from immature fruit of Ceratonia siliqua L. (carob) and in commercial carob syrup. Planta 92(1): 41-49, 1970

Dynamic changes in targeted phenolic compounds and antioxidant potency of carob fruit (Ceratonia siliqua L.) products during in vitro digestion. Lwt-Food Science and Technology 101: 269-275, 2019