+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Introgression of a Tombusvirus Resistance Locus from Nicotiana edwardsonii var. Columbia to N. clevelandii



Introgression of a Tombusvirus Resistance Locus from Nicotiana edwardsonii var. Columbia to N. clevelandii



Phytopathology 96(5): 453-459



ABSTRACT A new variety of Nicotiana, N. edwardsonii var. Columbia, was evaluated for its capacity to serve as a new source for virus resistance genes. Columbia was developed from a hybridization between N. glutinosa and N. clevelandii, the same parents used for the formation of the original N. edwardsonii. However, in contrast to the original N. edwardsonii, crosses between Columbia and either of its parents are fertile. Thus, the inheritance of virus resistance genes present in N. glutinosa could be characterized by using Columbia as a bridge plant in crosses with the susceptible parent, N. clevelandii. To determine how virus resistance genes would segregate in interspecific crosses between Columbia and N. clevelandii, we followed the fate of the N gene, a single dominant gene that specifies resistance to Tobacco mosaic virus (TMV). Our genetic evidence indicated that the entire chromosome containing the N gene was introgressed into N. clevelandii to create an addition line, designated N. clevelandii line 19. Although line 19 was homozygous for resistance to TMV, it remained susceptible to Tomato bushy stunt virus (TBSV) and Cauliflower mosaic virus (CaMV) strain W260, indicating that resistance to these viruses must reside on other N. glutinosa chromosomes. We also developed a second addition line, N. clevelandii line 36, which was homozygous for resistance to TBSV. Line 36 was susceptible to TMV and CaMV strain W260, but was resistant to other tombusviruses, including Cucumber necrosis virus, Cymbidium ringspot virus, Lettuce necrotic stunt virus, and Carnation Italian ringspot virus.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 004444828

Download citation: RISBibTeXText

PMID: 18944304

DOI: 10.1094/PHYTO-96-0453


Related references

Introgression of virus resistance genes from N-glutinosa to N-clevelandii. Phytopathology 94(6): S110-S110, 2004

Silencing of the N family of resistance genes in Nicotiana edwardsonii compromises the hypersensitive response to tombusviruses. Molecular Plant-Microbe Interactions 20(10): 1262-1270, 2007

Dissecting the hypersensitive response of Nicotiana edwardsonii to CaMV Resistance and necrosis are determined by two separate plant genes. Phytopathology 89(6 SUPPL ): S16-S17, 1999

Consistency of Nicotiana attenuata's herbivore- and jasmonate-induced transcriptional responses in the allotetraploid species Nicotiana quadrivalvis and Nicotiana clevelandii. Plant Physiology 135(1): 539-548, 2004

A gene from Nicotiana bigelovii selectively suppresses cell death induced during the hypersensitive response of Nicotiana edwardsonii to CaMV infection. Phytopathology 91(6 Supplement): S14, 2001

Coat protein mediated resistance to Plum Pox Virus in Nicotiana clevelandii and N. benthamiana. Plant Cell Reports 11(1): 30-33, 1992

Transfer of resistance to potato virus Y (PVY) from Nicotiana africana to Nicotiana tabacum: possible influence of tissue culture on the rate of introgression. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 110(4): 678-687, 2005

Temporal expression of PR-1 in Nicotiana edwardsonii A dominant Mendelian trait correlated with enhanced resistance to virus-induced necrotization and increases in salicylic acid levels. Free Radical Research 37(Supplement 2): 16, 2003

Iron containing inclusions in chloroplasts of Nicotiana clevelandii X Nicotiana glutinosa. I. X-ray microanalysis and ultrastructure. Zeitschrift fur Pflanzenphysiologie: 9 (2) 165-176, 1976

Experimental androgenesis in vitro in Nicotiana clevelandii Gray and Nicotiana sanderae hort. 1974

Composition of electron dense stroma inclusions in pro plastids of nicotiana clevelandii x nicotiana glutinosa. Cytologia 44(3): 581-584, 1979

Iron containing inclusion in chloroplasts of nicotiana clevelandii x nicotiana glutinosa part 1 x ray micro analysis and ultrastructure. Chromosoma 79(2): 165-176, 1976

Capsidiol an anti fungal compound produced in nicotiana tabacum and nicotiana clevelandii following infection with tobacco necrosis virus. Phytochemistry 14(2): 597, 1975

Transformation in Nicotiana edwardsonii. Biotechnology in agriculture and forestry2(22): 294-307, 1993

Transformation and regeneration of nicotiana edwardsonii. Plant Science 64(1): 67-78, 1989