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Isolation and analysis of Sox9 derived from yellow catfish (Pelteobagrus fulvidraco)



Isolation and analysis of Sox9 derived from yellow catfish (Pelteobagrus fulvidraco)



Journal of Agricultural Biotechnology 13(5): 620-623



Sox9 is one of important genes related with sex differentiation and determination. Two cDNAs encoding Sox9 were derived from yellow catfish (Pelteobagrus fulvidraco) brain, testis and ovary using RT-PCR. Sequence analysis revealed the ovarian Sox9a2 (GenBank accession No.AY684329) was different from brain and testis-derived Sox9a1 (GenBank accession No. AY684328), and the identity rate of cDNA was 77%, amino acids were 84%. It was confirmed that there were two genes for this expression.

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