Molecular identification of Populus tomentosa triploid clones by AFLP
H.X.aoLi; Zhou ChunJiang; Yue LiangSong
Journal of Beijing Forestry University 28(2): 9-14
Twelve Populus tomentosa triploids were identified by amplified fragment length polymorphism molecular technology using a Mse I + 3/EcoR I + 2 primer combinations. Twelve primers with high amplified bands were selected from 38 primer combinations, including primer M-CAT/E-TC with the highest ratio of polymorphic sites, as well as primer M-CTT/E-TT with the lowest ratio of polymorphic sites. The genetic relationships among the 12 materials were relatively close.