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Agrobacterium-mediated transformation for ureB gene from Helicobacter pylori in strawberry (Fragaria ananassa Duch.)



Agrobacterium-mediated transformation for ureB gene from Helicobacter pylori in strawberry (Fragaria ananassa Duch.)



Acta Agriculturae Zhejiangensis 18(3): 133-136



H. pylori ureB gene was cloned at the 5' end of the GUS reporter gene between the CaMV35S promoter and the NOS terminator of the plasmid pCAMBIA13011-ureB before introducing it to strawberry leaf discs by Agrobacterium-mediated transformation. After 2 times of selection in hygromycin culture, a total of 60 regenerated plants with hygromycin resistance were obtained, of which 50% were transgenic.

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Accession: 004506271

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