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Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay

Lugovskaya, N.N.; Scherbakov, A.V.; Yakovleva, A.S.; Tsyvanyuk, M.A.; Mudrak, N.S.; Drygin, V.V.; Borisov, A.V.

Journal of Virological Methods 135(2): 292-296

2006


ISSN/ISBN: 0166-0934
PMID: 16675032
DOI: 10.1016/j.jviromet.2006.03.019
Accession: 004510996

The recombinant antigen obtained by cloning and expressing two IBV nucleocapsid protein fragments (143-414 aa, 281-414 aa) in Escherichia coli was used for the detection of avian infectious bronchitis virus (IBV) specific antibodies in chicken sera by the indirect ELISA (rNpIBV-ELISA). As a result of testing 1524 serum samples the diagnostic sensitivity and specificity of rNpIBV-ELISA when comparing those of the routine whole IBV ELISA have been shown to be 93.81% and 87.36%, respectively. The agreement value was 91.5%.

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