Determination of eleutheroside E and eleutheroside B in rat plasma and tissue by high-performance liquid chromatography using solid-phase extraction and photodiode array detection
Feng, S.lan.; Hu, F.di.; Zhao, J.Xiong.; Liu, X.; Li, Y'un.
European Journal of Pharmaceutics and Biopharmaceutics Official Journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik E.V 62(3): 315-320
2005
ISSN/ISBN: 0939-6411 PMID: 16318914 DOI: 10.1016/j.ejpb.2005.09.007
Accession: 004511095
A HPLC method with photodiode array detection (PDA) was developed for the determination and a pharmacokinetic study of eleutheroside E (ELU E) and eleutheroside B (ELU B) in rat plasma and tissue following an eleutherococcus injection. The analysis was performed on a Kromasil C18 column, using water-acetonitrile as the gradient mobile phase and 0.8 mL/min flow rate. Detection wavelengths of ELU E and ELU B were 220 and 206 nm, respectively.